Purification and characterization of a 29 kDa poly(A)-binding protein from chickpea (Cicer arietinum) epicotyl.

نویسندگان

  • V Cheriyath
  • A Balasubrahmanyam
  • H C Kapoor
چکیده

A poly(A)-binding protein (PABP) with mol wt 29,000 has been purified from chickpea (Cicer arietinum) epicotyl by ammonium sulfate fractionation and Cibacron blue F3-GA chromatography, making a complex with poly(A) and elution of PABP-poly(A) complex at 45 degrees C from oligo d(T)-cellulose. The elution pattern and binding properties show that the purified protein is different from the PABP (mol. wt 72,000) reported earlier from our laboratory.

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عنوان ژورنال:
  • Indian journal of biochemistry & biophysics

دوره 38 4  شماره 

صفحات  -

تاریخ انتشار 2001